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Eurofins Genomics dna sequencing service
Dna Sequencing Service, supplied by Eurofins Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+sequencing+service/eurofins+genomics+mix2seq+sequencing+service/10__1042_slash_bcj20250321-163-8-11
Average 86 stars, based on 1 article reviews
dna sequencing service - by Bioz Stars, 2026-10
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Construct:

Article Title: Identification of essential residues for the biosynthesis of momilactone intermediate diterpene in wild rice <i>Oryza officinalis</i>
Article Snippet: .. Nucleotide sequences of all constructs were confirmed by DNA sequencing service (eurofins genomics, Tokyo, Japan). ..

DNA Sequencing:

Article Title: Identification of essential residues for the biosynthesis of momilactone intermediate diterpene in wild rice <i>Oryza officinalis</i>
Article Snippet: .. Nucleotide sequences of all constructs were confirmed by DNA sequencing service (eurofins genomics, Tokyo, Japan). ..

Article Title: Nkx2-1 and FoxE regionalize glandular (mucus-producing) and thyroid-equivalent traits in the endostyle of the chordate Oikopleura dioica.
Article Snippet: The endostyle is a ventral pharyngeal organ used for internal filter feeding of basal chordates and is considered homologous to the follicular thyroid of vertebrates.. It contains mucus-producing (glandular) and thyroidequivalent regions organized along the dorsoventral (DV) axis.. Although thyroid-related genes (Nkx2-1, FoxE, and thyroid peroxidase (TPO)) are known to be expressed in the endostyle, their roles in establishing regionalization within the organ have not been demonstrated.

Article Title: Ubiquity of methanogenic archaea in the trunk of coniferous and broadleaved tree species in a mountain forest.
Article Snippet: Acer pictum, Aesculus turbinata, Magnolia obovata, and Populus tremula.. Amplicon sequencing analysis of 16S rRNA genes showed that Methanobacteriaceae predominated the archaeal communities and Methanomassiliicoccaceae also inhabited some trees.. Real-time PCR analysis detected methanogenic archaeal mcrA genes from all the tree species, with a maximum of 107 copies g−1 dry wood.

Article Title: Assessing Olive Oil Quality Using Different DNA-Based Methods
Article Snippet: The amplification reaction took place in an AB2720 Thermocycler (Applied Biosystems, Waltham, MA, USA). .. The resulting amplicon was purified directly from the PCR reaction mixture using the QIAquick PCR purification kit (Cat. No. 28104, Qiagen, Milano, Italy) and sequenced on both strands using the same primer pairs used in the PCR step by the Eurofins Genomics DNA sequencing service ( https://www.eurofinsgenomics.eu , accessed on 13 October 2023). ..

Amplification:

Article Title: Nkx2-1 and FoxE regionalize glandular (mucus-producing) and thyroid-equivalent traits in the endostyle of the chordate Oikopleura dioica.
Article Snippet: The endostyle is a ventral pharyngeal organ used for internal filter feeding of basal chordates and is considered homologous to the follicular thyroid of vertebrates.. It contains mucus-producing (glandular) and thyroidequivalent regions organized along the dorsoventral (DV) axis.. Although thyroid-related genes (Nkx2-1, FoxE, and thyroid peroxidase (TPO)) are known to be expressed in the endostyle, their roles in establishing regionalization within the organ have not been demonstrated.

Article Title: Assessing Olive Oil Quality Using Different DNA-Based Methods
Article Snippet: The amplification reaction took place in an AB2720 Thermocycler (Applied Biosystems, Waltham, MA, USA). .. The resulting amplicon was purified directly from the PCR reaction mixture using the QIAquick PCR purification kit (Cat. No. 28104, Qiagen, Milano, Italy) and sequenced on both strands using the same primer pairs used in the PCR step by the Eurofins Genomics DNA sequencing service ( https://www.eurofinsgenomics.eu , accessed on 13 October 2023). ..

Plasmid Preparation:

Article Title: Nkx2-1 and FoxE regionalize glandular (mucus-producing) and thyroid-equivalent traits in the endostyle of the chordate Oikopleura dioica.
Article Snippet: The endostyle is a ventral pharyngeal organ used for internal filter feeding of basal chordates and is considered homologous to the follicular thyroid of vertebrates.. It contains mucus-producing (glandular) and thyroidequivalent regions organized along the dorsoventral (DV) axis.. Although thyroid-related genes (Nkx2-1, FoxE, and thyroid peroxidase (TPO)) are known to be expressed in the endostyle, their roles in establishing regionalization within the organ have not been demonstrated.

Isolation:

Article Title: Nkx2-1 and FoxE regionalize glandular (mucus-producing) and thyroid-equivalent traits in the endostyle of the chordate Oikopleura dioica.
Article Snippet: The endostyle is a ventral pharyngeal organ used for internal filter feeding of basal chordates and is considered homologous to the follicular thyroid of vertebrates.. It contains mucus-producing (glandular) and thyroidequivalent regions organized along the dorsoventral (DV) axis.. Although thyroid-related genes (Nkx2-1, FoxE, and thyroid peroxidase (TPO)) are known to be expressed in the endostyle, their roles in establishing regionalization within the organ have not been demonstrated.

Sequencing:

Article Title: Nkx2-1 and FoxE regionalize glandular (mucus-producing) and thyroid-equivalent traits in the endostyle of the chordate Oikopleura dioica.
Article Snippet: The endostyle is a ventral pharyngeal organ used for internal filter feeding of basal chordates and is considered homologous to the follicular thyroid of vertebrates.. It contains mucus-producing (glandular) and thyroidequivalent regions organized along the dorsoventral (DV) axis.. Although thyroid-related genes (Nkx2-1, FoxE, and thyroid peroxidase (TPO)) are known to be expressed in the endostyle, their roles in establishing regionalization within the organ have not been demonstrated.

Polymerase Chain Reaction:

Article Title: Ubiquity of methanogenic archaea in the trunk of coniferous and broadleaved tree species in a mountain forest.
Article Snippet: Acer pictum, Aesculus turbinata, Magnolia obovata, and Populus tremula.. Amplicon sequencing analysis of 16S rRNA genes showed that Methanobacteriaceae predominated the archaeal communities and Methanomassiliicoccaceae also inhabited some trees.. Real-time PCR analysis detected methanogenic archaeal mcrA genes from all the tree species, with a maximum of 107 copies g−1 dry wood.

Article Title: Assessing Olive Oil Quality Using Different DNA-Based Methods
Article Snippet: The amplification reaction took place in an AB2720 Thermocycler (Applied Biosystems, Waltham, MA, USA). .. The resulting amplicon was purified directly from the PCR reaction mixture using the QIAquick PCR purification kit (Cat. No. 28104, Qiagen, Milano, Italy) and sequenced on both strands using the same primer pairs used in the PCR step by the Eurofins Genomics DNA sequencing service ( https://www.eurofinsgenomics.eu , accessed on 13 October 2023). ..

Purification:

Article Title: Assessing Olive Oil Quality Using Different DNA-Based Methods
Article Snippet: The amplification reaction took place in an AB2720 Thermocycler (Applied Biosystems, Waltham, MA, USA). .. The resulting amplicon was purified directly from the PCR reaction mixture using the QIAquick PCR purification kit (Cat. No. 28104, Qiagen, Milano, Italy) and sequenced on both strands using the same primer pairs used in the PCR step by the Eurofins Genomics DNA sequencing service ( https://www.eurofinsgenomics.eu , accessed on 13 October 2023). ..



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(A) Architecture of the recombinase-based memory switch. The input module (pSwitch) contains IPTG- and arabinose-inducible promoters driving the expression of HbiF and FimE, respectively. The output module (pReporter) contains recombinase recognition sites flanking a constitutive promoter (Ptrc*) controlling RFP and GFP expression. Site-specific <t>DNA</t> inversion between the inverted repeats (IRL/IRR) reconfigures the recombination sites, reversing the promoter orientation and thereby toggling expression between RFP and GFP . (B) Flow cytometry analysis of cells initialized in the Green-On state and analyzed on day 3. The percentages of Red, uncommitted, and Green cells are reported as indicated. Red circles indicate the effect of recombinase leakiness and non-orthogonality. (C) Flow cytometry analysis of cells initialized in the Green-On state and switched to the Red-On state on day 3. (D) Flow cytometry analysis of cells initialized in the Red-On state and analyzed on day 3. (E) Flow cytometry analysis of cells initialized in the Red-On state and switched to the Green-On state on day 3.
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(A) Architecture of the recombinase-based memory switch. The input module (pSwitch) contains IPTG- and arabinose-inducible promoters driving the expression of HbiF and FimE, respectively. The output module (pReporter) contains recombinase recognition sites flanking a constitutive promoter (Ptrc*) controlling RFP and GFP expression. Site-specific <t>DNA</t> inversion between the inverted repeats (IRL/IRR) reconfigures the recombination sites, reversing the promoter orientation and thereby toggling expression between RFP and GFP . (B) Flow cytometry analysis of cells initialized in the Green-On state and analyzed on day 3. The percentages of Red, uncommitted, and Green cells are reported as indicated. Red circles indicate the effect of recombinase leakiness and non-orthogonality. (C) Flow cytometry analysis of cells initialized in the Green-On state and switched to the Red-On state on day 3. (D) Flow cytometry analysis of cells initialized in the Red-On state and analyzed on day 3. (E) Flow cytometry analysis of cells initialized in the Red-On state and switched to the Green-On state on day 3.
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(A) Architecture of the recombinase-based memory switch. The input module (pSwitch) contains IPTG- and arabinose-inducible promoters driving the expression of HbiF and FimE, respectively. The output module (pReporter) contains recombinase recognition sites flanking a constitutive promoter (Ptrc*) controlling RFP and GFP expression. Site-specific <t>DNA</t> inversion between the inverted repeats (IRL/IRR) reconfigures the recombination sites, reversing the promoter orientation and thereby toggling expression between RFP and GFP . (B) Flow cytometry analysis of cells initialized in the Green-On state and analyzed on day 3. The percentages of Red, uncommitted, and Green cells are reported as indicated. Red circles indicate the effect of recombinase leakiness and non-orthogonality. (C) Flow cytometry analysis of cells initialized in the Green-On state and switched to the Red-On state on day 3. (D) Flow cytometry analysis of cells initialized in the Red-On state and analyzed on day 3. (E) Flow cytometry analysis of cells initialized in the Red-On state and switched to the Green-On state on day 3.
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(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
Dna Sequencing Service, supplied by Eurofins Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+sequencing+service/eurofins+genomics+mix2seq+sequencing+service/10__1042_slash_bcj20250321-163-8-11
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(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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Image Search Results


(A) Architecture of the recombinase-based memory switch. The input module (pSwitch) contains IPTG- and arabinose-inducible promoters driving the expression of HbiF and FimE, respectively. The output module (pReporter) contains recombinase recognition sites flanking a constitutive promoter (Ptrc*) controlling RFP and GFP expression. Site-specific DNA inversion between the inverted repeats (IRL/IRR) reconfigures the recombination sites, reversing the promoter orientation and thereby toggling expression between RFP and GFP . (B) Flow cytometry analysis of cells initialized in the Green-On state and analyzed on day 3. The percentages of Red, uncommitted, and Green cells are reported as indicated. Red circles indicate the effect of recombinase leakiness and non-orthogonality. (C) Flow cytometry analysis of cells initialized in the Green-On state and switched to the Red-On state on day 3. (D) Flow cytometry analysis of cells initialized in the Red-On state and analyzed on day 3. (E) Flow cytometry analysis of cells initialized in the Red-On state and switched to the Green-On state on day 3.

Journal: bioRxiv

Article Title: Engineering Persistent and Rewritable Genetic Memory in Bacteria through Multiscale Plasmid Competition

doi: 10.64898/2026.09.18.752696

Figure Lengend Snippet: (A) Architecture of the recombinase-based memory switch. The input module (pSwitch) contains IPTG- and arabinose-inducible promoters driving the expression of HbiF and FimE, respectively. The output module (pReporter) contains recombinase recognition sites flanking a constitutive promoter (Ptrc*) controlling RFP and GFP expression. Site-specific DNA inversion between the inverted repeats (IRL/IRR) reconfigures the recombination sites, reversing the promoter orientation and thereby toggling expression between RFP and GFP . (B) Flow cytometry analysis of cells initialized in the Green-On state and analyzed on day 3. The percentages of Red, uncommitted, and Green cells are reported as indicated. Red circles indicate the effect of recombinase leakiness and non-orthogonality. (C) Flow cytometry analysis of cells initialized in the Green-On state and switched to the Red-On state on day 3. (D) Flow cytometry analysis of cells initialized in the Red-On state and analyzed on day 3. (E) Flow cytometry analysis of cells initialized in the Red-On state and switched to the Green-On state on day 3.

Article Snippet: DNA sequencing used Plasmidsaurus DNA whole plasmid sequencing service.

Techniques: Expressing, Flow Cytometry, Analysis

(A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus DNA ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense sequence (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).

Journal: Bio-protocol

Article Title: Plasmid Curing of Pseudoalteromonas haloplanktis TAC125 Using Homologous Recombination and PTasRNA Gene Silencing

doi: 10.21769/BioProtoc.5687

Figure Lengend Snippet: (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus DNA ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense sequence (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).

Article Snippet: DNA sequencing service (e.g., Eurofins Genomics, Ebersberg, Germany) 7.

Techniques: Agarose Gel Electrophoresis, Plasmid Preparation, Negative Control, Cloning, Sequencing, Marker